ionized calcium Search Results


anti iba1  (Alomone Labs)
94
Alomone Labs anti iba1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Anti Iba1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti iba1/product/Alomone Labs
Average 94 stars, based on 1 article reviews
anti iba1 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
anti iba 1  (ProSci Incorporated)
92
ProSci Incorporated anti iba 1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Anti Iba 1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti iba 1/product/ProSci Incorporated
Average 92 stars, based on 1 article reviews
anti iba 1 - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
adapter molecule  (ProSci Incorporated)
93
ProSci Incorporated adapter molecule
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Adapter Molecule, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adapter molecule/product/ProSci Incorporated
Average 93 stars, based on 1 article reviews
adapter molecule - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
ionized calcium-binding adaptor molecule 1 (iba1  (Arigo Biolaboratories)
90
Arigo Biolaboratories ionized calcium-binding adaptor molecule 1 (iba1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Ionized Calcium Binding Adaptor Molecule 1 (Iba1, supplied by Arigo Biolaboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionized calcium-binding adaptor molecule 1 (iba1/product/Arigo Biolaboratories
Average 90 stars, based on 1 article reviews
ionized calcium-binding adaptor molecule 1 (iba1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
monoclonal aif19mouse anti-iba1  (Merck KGaA)
90
Merck KGaA monoclonal aif19mouse anti-iba1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Monoclonal Aif19mouse Anti Iba1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal aif19mouse anti-iba1/product/Merck KGaA
Average 90 stars, based on 1 article reviews
monoclonal aif19mouse anti-iba1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
monoclonal rabbit anti–ionized calcium-binding adapter molecule 1  (FUJIFILM)
90
FUJIFILM monoclonal rabbit anti–ionized calcium-binding adapter molecule 1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Monoclonal Rabbit Anti–Ionized Calcium Binding Adapter Molecule 1, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal rabbit anti–ionized calcium-binding adapter molecule 1/product/FUJIFILM
Average 90 stars, based on 1 article reviews
monoclonal rabbit anti–ionized calcium-binding adapter molecule 1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
guinea pig ionized calcium binding adaptor protein 1 antibody  (Synaptic Systems)
90
Synaptic Systems guinea pig ionized calcium binding adaptor protein 1 antibody
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Guinea Pig Ionized Calcium Binding Adaptor Protein 1 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/guinea pig ionized calcium binding adaptor protein 1 antibody/product/Synaptic Systems
Average 90 stars, based on 1 article reviews
guinea pig ionized calcium binding adaptor protein 1 antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ionized calcium  (Blackwell Science Ltd)
90
Blackwell Science Ltd ionized calcium
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Ionized Calcium, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionized calcium/product/Blackwell Science Ltd
Average 90 stars, based on 1 article reviews
ionized calcium - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti-ionizing calcium-binding adapter molecule 1  (Synaptic Systems)
90
Synaptic Systems rabbit anti-ionizing calcium-binding adapter molecule 1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Rabbit Anti Ionizing Calcium Binding Adapter Molecule 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-ionizing calcium-binding adapter molecule 1/product/Synaptic Systems
Average 90 stars, based on 1 article reviews
rabbit anti-ionizing calcium-binding adapter molecule 1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
dual channel platelet ionized calcium aggregometer  (Chrono-log corporation)
90
Chrono-log corporation dual channel platelet ionized calcium aggregometer
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Dual Channel Platelet Ionized Calcium Aggregometer, supplied by Chrono-log corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dual channel platelet ionized calcium aggregometer/product/Chrono-log corporation
Average 90 stars, based on 1 article reviews
dual channel platelet ionized calcium aggregometer - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
primary antibodies against ionized calcium-binding adaptor molecule 1  (Merck KGaA)
90
Merck KGaA primary antibodies against ionized calcium-binding adaptor molecule 1
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Primary Antibodies Against Ionized Calcium Binding Adaptor Molecule 1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against ionized calcium-binding adaptor molecule 1/product/Merck KGaA
Average 90 stars, based on 1 article reviews
primary antibodies against ionized calcium-binding adaptor molecule 1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ionized calcium analyzer  (LabCorp)
90
LabCorp ionized calcium analyzer
PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and <t>Iba1</t> IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.
Ionized Calcium Analyzer, supplied by LabCorp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionized calcium analyzer/product/LabCorp
Average 90 stars, based on 1 article reviews
ionized calcium analyzer - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and Iba1 IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.

Journal: The Journal of Cell Biology

Article Title: Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord

doi: 10.1083/jcb.201607048

Figure Lengend Snippet: PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and Iba1 IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.

Article Snippet: Slices were permeabilized and blocked in PBS 0.1% Triton X-100 and 0.25% fish gelatin and incubated with primary antibodies (anti-GlyR α1 [1:400; rabbit]; anti-GABA A R γ2 [1:50; Alomone Labs], anti-gephyrin [1:400; mAb7a; Synaptic Systems], anti-Cox-2 [1:200; M-19; Santa Cruz Biotechnology], anti-Iba1 [1:400; Wako], and anti-EP2R [Alomone Labs]).

Techniques: Labeling, Fluorescence, Imaging